HumanKine® recombinant human DKK-1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

7-70 ng/mL

Species Reactivity

human

Purity

>95%

Publications

1

Cat no : HZ-1314

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1

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Validation Data Gallery

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  Purity of recombinant human DKK-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human DKK-1 (HZ-1314) inhibits dose-dependent luciferase production by Wnt3A in a HEK293 reporter cell line. Luciferase assay production was assessed by One-Step ™ luciferase assay kit. Hek293 cells were treated with increasing amounts of DKK-1 as well as 150ng/mL of recombinant Wnt3A (HZ-1296) for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 7-70ng/mL.

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Technical Specifications

• GeneID: 22943
• Species: Human
• Expression: HEK293
• Activity(Primary): 7-70 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: O94907
• Molecular Mass: 37-51 kDa reduced, 35-49 non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

DKK-1 (Dickkopf WNT signaling pathway inhibitor 1) is a member of the DKK protein family. Other members of this family include DKK-2, DKK-3, and DKK-4. The DKK family proteins share 35-40 % homology between various members. Recombinant human DKK-1 expressed in HEK293 cells is a 35-45kDa glycosylated protein. It is a secreted protein that functions as a negative regulator of Wnt/β-catenin signaling pathway. Wnt/ β-catenin signaling pathway involves binding of the Wnt ligands to the seven-transmembrane receptor, Frizzled, and the coreceptor lipoprotein-related protein 5 and 6 (LRP5/6). DKK1 forms a ternary complex with LRP5/6 and another receptor, Kremen, followed by endocytosis of this complex and removal of LRP5/6 from the cell surface. It has been suggested that DKK-1 permits anterior development by inhibiting Wnt/ β-catenin signaling, which is essential for posterior patterning in vertebrates. The balance between Wnt signaling and DKK-1 inhibition is critical for bone formation and homeostasis and insufficient or excess DKK-1 activity in bone results in increased or decreased bone density, respectively. (PMID: 17143291; 12050670; 10570958)

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1

Publications

Species	Title
human	Cell Stem Cell; Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.; Authors - Anindita Sarkar