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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human CNTF protein

Activity(Primary)

2.75-14 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1331

Synonyms

Ciliary Neurotrophic Factor

Validation Data Gallery

  • Purity of recombinant human CNTF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human CNTF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human CNTF (Cat no: HZ-1331) induces dose-dependent release of Heptoglobin in the HEPG2 hepatocellular carcinoma cell line in the presence of 200 ng/mL CNTFR. HEPG2 cells were treated with increasing concentration of recombinant CNTF for 16 hours before supernatant collection. The supernatant was tested for Haptoglobin via ELISA kit (KE00148). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2.75-14 ng/mL.

    Recombinant human CNTF (Cat no: HZ-1331) induces dose-dependent release of Heptoglobin in the HEPG2 hepatocellular carcinoma cell line in the presence of 200 ng/mL CNTFR. HEPG2 cells were treated with increasing concentration of recombinant CNTF for 16 hours before supernatant collection. The supernatant was tested for Haptoglobin via ELISA kit (KE00148). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2.75-14 ng/mL.

Technical Specifications

GeneID 1270
Species Human
Expression HEK293
Activity(Primary) 2.75-14 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP26441
Molecular Mass 23-27 kDa reduced and non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

CNTF or ciliary neurotrophic factor, is a member of the IL-6 family of cytokines alongside LIF, OSM, and cardiotropin-1. It promotes gene expression, cell survival, and differentiation of sensory, ciliary, and motor neurons. It promotes rod and cone photoreceptor regeneration in response to retinal injury and degeneration. It is also thought to control B cell differentiation in the bone marrow. CNTF can be used to maintain neural stem cells in the pluripotent state, or it can promote their further differentiation into glial precursor cells, astrocytes, and oligodendrocytes (PMID: 30504707, 21912637, 26487326).

Synonyms

Ciliary Neurotrophic Factor

Publications

SpeciesTitle

ACS Biomater Sci Eng

Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat

Authors - Hailiang Xu
View Article