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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Betacellulin protein

Activity(Primary)

1 - 10 ng/mL in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1339

Synonyms

BTC

Validation Data Gallery

  • Purity of recombinant human Betacellulin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Betacellulin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human Betacellulin (HZ-1339) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10ng/mL.

    Recombinant human Betacellulin (HZ-1339) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10ng/mL.

  • Proteintech Betacellulin (HZ-1339) demonstrates nearly 50-fold decrease in EC50 and much higher fold induction compared to leading competitor A. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium.Cell number was quantitatively assessed by PrestoBlue® HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.

    Proteintech Betacellulin (HZ-1339) demonstrates nearly 50-fold decrease in EC50 and much higher fold induction compared to leading competitor A. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium.Cell number was quantitatively assessed by PrestoBlue® HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.

  • Proteintech Betacellulin (HZ-1339) demonstrates more than 100-fold decrease in EC50 and much higher fold induction compared to leading competitor B. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. Cell number was quantitatively assessed by PrestoBlue® HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.

    Proteintech Betacellulin (HZ-1339) demonstrates more than 100-fold decrease in EC50 and much higher fold induction compared to leading competitor B. NIH/3T3 cells were serum starved during treatment with increasing concentrations of recombinant human Betacellulin for 72hrs in defined medium. Cell number was quantitatively assessed by PrestoBlue® HS Cell Viability Reagent. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 1 - 10 ng/mL.

Technical Specifications

GeneID 685
Species Human
Expression HEK293
Activity(Primary) 1 - 10 ng/mL in NIH 3T3 cells in defined media
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP35070
Molecular Mass 12 to 25 kDa reduced, 11 to 23 kDa non-reduced, monomer, glycosylated
Formulation 1 x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family of proteins, which play a critical role in cell growth, differentiation, and repair. It is initially synthesized as a membrane-bound precursor, later cleaved to release its active soluble form (PMID 27635238). BTC is known to bind to and activate the ErbB receptor family, particularly ErbB1 (also known as EGFR) and ErbB4, triggering a cascade of intracellular signaling pathways that regulate cell proliferation and survival (PMID 24440602). BTC is highly expressed in various tissues, including the pancreas, where it promotes the growth and regeneration of insulin-producing beta cells, suggesting a potential role in diabetes treatment (PMID 33553143). Due to its wide-ranging effects, betacellulin has been studied for therapeutic applications in regenerative medicine and cancer (PMID 31678994, 12704384).

Synonyms

BTC