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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 2 protein- GMP grade

EC50

0.018-0.18 ng/mL

Specific Activity

minimally 2.00 x 107 IU/mg, typically 3.52 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1092-GMP

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

Validation Data Gallery

  • Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human TGF beta 2 (HZ-1092-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.018-0.18 ng/mL.

    GMP Recombinant human TGF beta 2 (HZ-1092-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.018-0.18 ng/mL.

Technical Specifications

GeneID 7042
Species Human
Expression HEK293
EC50 0.018-0.18 ng/mL
Specific Activityminimally 2.00 x 107 IU/mg, typically 3.52 x 107 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP61812
Molecular Mass 13 kDa reduced, 23 kDa non-reduced, homodimer, non-glycosylated
Formulation 50 mM NaOAc pH 3.7, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 20% ethanol + 50 mM NaOAc + 75 mM HOAc. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Transforming growth factor beta-2 (TGFB2) belongs to the TGF-beta family. TGFB2 is a secreted protein known as a cytokine that plays a vital role during embryonic development (PMID 19289080). TGFB2 regulates cell proliferation, differentiation, adhesion, migration by transducing the signal through transmembrane receptors type I and type II (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins (PMID 18313409). Disruption of the TGFB/SMAD pathway has been implicated in many cancers types. TGFB2 has suppressive effects on interleukin-2 dependent T-cell growth (PMID 12064833). The TGFβ2 protein plays a role in the blood vessels formation, the regulation of muscle tissue and body fat development. TGFβ-2 is especially abundant in tissues related to the skeleton, where it helps regulate bone growth and form the extracellular matrix in the spaces between cells.

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2