Phospho-Marcks Polyclonal antibody
Phospho-Marcks Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB,ELISA
Conjugate
Unconjugated
Cat no : 10018-3-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Mouse J774 Macrophage cellss-treated with PMA, HeLa cells, Mouse J774 Macrophage cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 5 publications below |
Product Information
10018-3-AP targets Phospho-Marcks in WB,ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | myristoylated alanine rich protein kinase C substrate |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC046601 |
Gene symbol | Marcks |
Gene ID (NCBI) | 17118 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcks with S152/S163 phosphorylated and phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-Marcks antibody 10018-3-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Cell Physiol Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability. | ||
J Physiol 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase. | ||
J Neurosci NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia. | ||
Proc Natl Acad Sci U S A MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability. |