Phospho-Marcks Polyclonal antibody

Phospho-Marcks Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB,ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10018-3-AP

Synonyms

Macs, Marcks, p marcks, phospho-Marcks, phosphorylation Marcks, PKCSL

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Validation Data Gallery

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  WB analysis of Mouse J774 Macrophage cells using 10018-3-AP

  WB result of phospho-Marcks antibody (10018-3-AP, 1:1500) with Mouse J774 Macrophage cells-treated with PMA.

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  WB analysis of HeLa using 10018-3-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10018-3-AP (Marcks Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Mouse J774 Macrophage cellss-treated with PMA, HeLa cells, Mouse J774 Macrophage cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below

Product Information

10018-3-AP targets Phospho-Marcks in WB,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myristoylated alanine rich protein kinase C substrate
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 32 kDa
• GenBank accession number: BC046601
• Gene symbol: Marcks
• Gene ID (NCBI): 17118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcks with S152/S163 phosphorylated and phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated.

Publications

Species	Application	Title
human	WB	Open Virol J; HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.; Authors - Fiala Milan M
rat	WB	Am J Physiol Cell Physiol; Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.; Authors - Tinsley John H JH
rat	WB	J Physiol; 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.; Authors - Peng Ying-Jie YJ
rat	WB	J Neurosci; NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.; Authors - Peng Y-J YJ
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY