Phospho-Marcksl1 (Ser93/104) Polyclonal antibody

• 

  WB analysis using 10018-3-AP

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10018-3-AP (Phospho-Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

Phospho-Marcksl1 (Ser93/104) Polyclonal Antibody for WB, ELISA

Cat No. 10018-3-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (3)

Applications

WB, ELISA

Synonyms

Phospho-Marcksl1, MARCKS, MARCKS-like 1, Myristoylated alanine-rich C-kinase substrate, Marcksl1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

10018-3-AP targets Phospho-Marcksl1 (Ser93/104) in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myristoylated alanine rich protein kinase C substrate
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC046601
• Gene Symbol: Marcks
• Gene ID (NCBI): 17118
• RRID: AB_2297345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P26645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.

Publications

Species	Application	Title
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
rat	WB	J Neurosci; NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.; Authors - Peng Y-J YJ
rat	WB	J Physiol; 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.; Authors - Peng Ying-Jie YJ
rat	WB	Am J Physiol Cell Physiol; Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.; Authors - Tinsley John H JH
human	WB	Open Virol J; HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.; Authors - Fiala Milan M