Phospho-TDP43 (Ser409/410) Recombinant antibody

Phospho-TDP43 (Ser409/410) Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

6M10

Reviews (1)

Cat no : 80007-1-RR

Synonyms

ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

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Validation Data Gallery

• 

  WB analysis of HeLa using 80007-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR

  IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

• 

  IHC staining of human brain using 80007-1-RR

  It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: frontal cortex of FTLD-TDP type B case, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Product Information

80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_2882937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Publications

Species	Application	Title
	WB	Biochim Biophys Acta Mol Basis Dis; Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.; Authors - Josephine C Esposto
human	WB,IF	bioRxiv; TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis; Authors - Baggio A Evangelista
mouse	IF	Res Sq; Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence; Authors - Joy Mitra
mouse	WB	Mol Neurobiol; A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis; Authors - Natasha Vassileff
mouse	IF	J Exp Med; A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice; Authors - David Brenner
human	IF,IHC	Brain Pathol; Detection of pTDP-43 via routine muscle biopsy: A promising diagnostic biomarker for amyotrophic lateral sclerosis; Authors - Qi-Jie Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Silvia (Verified Customer) (08-21-2020)

Excellent phospho-specific TDP-43 antibody. Tested by IHC in ethanol and NBF fixed FTLD-TDP brains and ALS spinal cord. The antibody works well with citrate buffer and FA as antigen retrieval. The dilutions that worked better showing high specificity and low background ranged from 1,000-5,000.Works great also by western blot at dilutions 1:1,000-1:2,000.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000)
• Cell Tissue Type: human cortex and spinal cord

Phospho-TDP43 (Ser409/410) Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000) dilution) in human cortex and spinal cord (Cat no:80007-1-RR)