Phospho-TDP43 (Ser409/410) Recombinant antibody, PBS Only (Capture)

Phospho-TDP43 (Ser409/410) Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6M10

Cat no : 80007-1-PBS

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

---

Validation Data Gallery

• 

  WB analysis using 80007-1-RR (same clone as 80007-1-PBS)

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

  It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

  IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array in cell lysate using MP00589-1

  Cytometric bead array in cell lysate using MP00589-1, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 81350-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

• 

  Cytometric bead array in cell lysate using MP00589-2

  Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

---

Product Information

80007-1-PBS targets Phospho-TDP43 (Ser409/410) as part of a matched antibody pair:

MP00589-1: 80007-1-PBS capture and 81350-1-PBS detection (validated in Cytometric bead array)

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in N/A)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.