peroxiredoxin 2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10545-2-PBS

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

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Validation Data Gallery

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  WB analysis using 10545-2-AP (same clone as 10545-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  IP result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10545-2-AP (same clone as 10545-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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Product Information

10545-2-PBS targets peroxiredoxin 2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: peroxiredoxin 2 fusion protein Ag0835
• Full Name: peroxiredoxin 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003022
• Gene Symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)