Pan-PAX Polyclonal antibody

Pan-PAX Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21383-1-AP

Synonyms

PAX-8, PAX8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8

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Validation Data Gallery

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  WB analysis of SKOV-3 using 21383-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21383-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 21383-1-AP

  IP result of anti-Pan-PAX (IP:21383-1-AP, 3ug; Detection:21383-1-AP 1:800) with SKOV-3 cells lysate 2500ug.

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  IHC staining of human ovary cancer using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 21383-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Pan-PAX antibody (21383-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, A2780 cells
• Positive IP detected in: SKOV-3 cells
• Positive IHC detected in: human ovary cancer tissue, human kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

21383-1-AP targets Pan-PAX in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pan-PAX fusion protein Ag16018
• Full Name: paired box 8
• Calculated Molecular Weight: 450 aa, 48 kDa
• Observed Molecular Weight: 36-48 kDa
• GenBank Accession Number: BC001060
• Gene Symbol: PAX8
• Gene ID (NCBI): 7849
• RRID: AB_10733094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.

Publications

Species	Application	Title
mouse	IF	Life Sci; Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.; Authors - Qiwang Ma
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	IHC	Am J Surg Pathol; PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.; Authors - Tong Guo-Xia GX
mouse	IHC	Endocrine; PMID: 32281047; Authors - Mika Shimamura
pig	IF	PLoS One; PMID: 28231273; Authors - Sergio E Palma-Vera