p70(S6K) Monoclonal antibody, PBS Only (Capture)

p70(S6K) Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11C6

Cat no : 66638-1-PBS

Synonyms

Phospho-p70(S6K) (Thr389), p70 S6K, p70 S6 kinase, RPS6KB1, p70 S6K 1

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66638-1-Ig (same clone as 66638-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66638-1-Ig (same clone as 66638-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66638-1-Ig (same clone as 66638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66638-1-Ig (same clone as 66638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50673-1

  Cytometric bead array standard curve of MP50673-1, p70(S6K) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66638-1-PBS. Detection antibody: 66638-2-PBS. Standard:Ag5883. Range: 1.563-100 ng/mL.

---

Product Information

66638-1-PBS targets p70(S6K) as part of a matched antibody pair:

MP50673-1: 66638-1-PBS capture and 66638-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: p70(S6K) fusion protein Ag5883
• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-75 kDa
• GenBank Accession Number: BC053365
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.