p70(S6K) Polyclonal antibody

p70(S6K) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26587-1-AP

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6k, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

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Validation Data Gallery

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  WB analysis of MCF-7 using 26587-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26587-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26587-1-AP

  IP result of anti-p70(S6K) (IP:26587-1-AP, 4ug; Detection:26587-1-AP 1:300) with A549 cells lysate 1600ug.

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  IHC staining of human lung cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26587-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26587-1-AP (p70(S6K) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 26587-1-AP is p70(S6K) Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p70(S6K) fusion protein Ag24073
• Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 60-75 kDa
• GenBank Accession Number: BC053365
• Gene Symbol: p70 S6K
• Gene ID (NCBI): 6198
• RRID: AB_2880565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.

Publications

Species	Application	Title
pig	WB	Am J Physiol Endocrinol Metab; Prematurity blunts the feeding-induced stimulation of translation initiation signaling and protein synthesis in muscle of neonatal piglets.; Authors - Jane K Naberhuis
mouse	WB	J Agric Food Chem; Selenomethionine Promotes Milk Protein and Fat Synthesis and Proliferation of Mammary Epithelial Cells through the GPR37-mTOR-S6K1 Signaling; Authors - Jinlong Zhang