p57Kip2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-20 ng/mL

Cat No : MP00884-4

Print datasheet

Synonyms

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP00884-4

  Sandwich ELISA standard curve of MP00884-4, Human p57Kip2 Recombinant Matched Antibody Pair - PBS only. 83966-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19849. 83966-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP00884-4

  The mean p57Kip2 concentration was determined to be 45.59 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

---

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00884-4 targets p57Kip2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: p57Kip2 Recombinant antibody, PBS Only (Capture) 83966-2-PBS (241025B6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: p57Kip2 Recombinant antibody, PBS Only (Detector) 83966-1-PBS (241025A8). 100 μg. Concentration 1 mgl/ml.

Alternative p57Kip2 matched antibody pairs: MP00884-1, MP00884-2, MP00884-3

Matched pair protein standard: Ag19849

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 83966-2-PBS
• Tested Reactivity: human
• Immunogen: p57Kip2 fusion protein Ag19849
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• Synonyms: CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C
• GenBank accession number: BC067842
• Gene symbol: p57 Kip2/CDKN1C
• Uniprot: P49918
• Gene ID (NCBI): 1028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 83966-1-PBS
• Tested Reactivity: human
• Immunogen: p57Kip2 fusion protein Ag19849
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• Synonyms: CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C
• GenBank accession number: BC067842
• Gene symbol: p57 Kip2/CDKN1C
• Uniprot: P49918
• Gene ID (NCBI): 1028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.