ChromoTek p53 N-term-Trap Agarose, kit

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

Specificity

p53

Reactivity

Human

Applications

IP, CoIP

Conjugate

Agarose

Type

Nanobody

Cat no : ptak

Synonyms

p53 N-term-Trap, p53 Trap, p53-Trap,Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Product Information

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

DescriptionThe p53-N-term-Trap Agarose Kit contains p53-N-term-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of p53.

• Pull down of human p53 isoforms alpha, beta and gamma with p53-Trap-N-term

• Immunoprecipitation of the human p53 proteome with complementary p53-C-term- and p53-N-term-Traps, e.g. p53 isoforms and variants

• No heavy & light antibody chains in your downstream application

• Short incubation time (60 min at +4 °C)

ApplicationsIP, CoIP
Specificity/Targetp53 (isoforms: alpha, beta, and gamma); epitope 1-81 aa
Tested ReactivityHuman
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Type Nanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe p53-N-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631378
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
p53 N-term-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution