AMACR/p504S Polyclonal antibody

AMACR/p504S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15918-1-AP

Synonyms

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, AMACR/p504S, CBAS4, p504S, RACE

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Validation Data Gallery

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  WB analysis using 15918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15918-1-AP (AMACR/p504S antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

15918-1-AP targets AMACR/p504S in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMACR/p504S fusion protein Ag8720
• Full Name: alpha-methylacyl-CoA racemase
• Calculated Molecular Weight: 382 aa, 42 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC009471
• Gene Symbol: AMACR
• Gene ID (NCBI): 23600
• RRID: AB_11126973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and it can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IHC	Front Cell Dev Biol; SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer.; Authors - Zhao-Ming Xiao
human	IHC	Oncol Lett; The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.; Authors - Daijun Yang
human	IHC	Andrologia; Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.; Authors - Xiqi Ding
human	WB,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham