p38 MAPK Recombinant antibody

p38 MAPK Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230263H7

Cat no : 80821-3-RR

Synonyms

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

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Validation Data Gallery

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  WB analysis of mouse brain using 80821-3-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 80821-3-RR

  IP result of anti-p38 MAPK (IP:80821-3-RR, 4ug; Detection:80821-3-RR 1:2000) with HEK-293 cells lysate 1575 ug.

• 

  FC experiment of HeLa using 80821-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of Jurkat using 80821-3-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 80821-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80821-3-RR against Human p38 MAPK were performed. The affinity constant is 1.04 nM.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80821-3-RR targets p38 MAPK in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: p38 MAPK fusion protein Ag5115
• Full Name: mitogen-activated protein kinase 14
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC031574
• Gene Symbol: p38 MAPK
• Gene ID (NCBI): 1432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.