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p38 MAPK Polyclonal antibody, PBS Only

p38 MAPK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30382-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p38 MAPK Polyclonal antibody (30382-1-AP)

    WB analysis using 30382-1-AP (same clone as 30382-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30382-1-AP (p38 MAPK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p38 MAPK Polyclonal antibody (30382-1-AP)

    IF Staining of HepG2 using 30382-1-AP (same clone as 30382-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using p38 MAPK antibody (30382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30382-1-PBS in a different storage buffer formulation.

Product Information

30382-1-PBS targets p38 MAPK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p38 MAPK fusion protein Ag33174
Full Name mitogen-activated protein kinase 14
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.