p130Cas / BCAR1 Monoclonal antibody, PBS Only

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11C10

Cat no : 67215-1-PBS

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

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Validation Data Gallery

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  WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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Product Information

67215-1-PBS targets p130Cas / BCAR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: p130Cas / BCAR1 fusion protein Ag10604
• Full Name: breast cancer anti-estrogen resistance 1
• Calculated Molecular Weight: 870 aa, 93 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC062556
• Gene Symbol: BCAR1
• Gene ID (NCBI): 9564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.