nNOS Polyclonal antibody, PBS Only

nNOS Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18984-1-PBS

Synonyms

---

Validation Data Gallery

• 

  IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18984-1-AP (same clone as 18984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

---

Product Information

18984-1-PBS targets nNOS in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nitric oxide synthase 1 (neuronal)
• Calculated Molecular Weight: 161 kDa
• GenBank Accession Number: NM_000620
• Gene Symbol: nNOS
• Gene ID (NCBI): 4842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1). The antibody can recognize isoform 1,2,4 of nNOS.