ChromoTek mNeonGreen Chromobody® plasmid (mCherry)

DNA plasmid encoding for anti-mNeonGreen VHH (Nanobody) fused to fluorescent protein mCherry.

Reactivity

mNeonGreen

Applications

IF, Live Cell Imaging

Vector Type

Mammalian expression vector

Reporter/Conjugate

mCherry

Cat no : mcr

Synonyms

Chromobody, mNeonGreen Chromobody, mNeonGreen Live Cell, Live-Cell Imaging, Live Cell Imaging, Live Cell, Live-Cell

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Validation Data Gallery

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  Fluorescence microscopy of HeLa cells, transiently expressing Vimentin-mNeonGreen (green) and the mNeonGreen-Chromobody-(mCherry) (red). Nuclei are stained with DAPI (cyan). Green and red fluorescent signals co-localize in co-transfected cells, indicating enrichment of the Chromobody at the mNeonGreen-positive structures. Confocal images were were acquired with a 63x oil objective and post-processed.

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Product Information

DNA plasmid encoding for anti-mNeonGreen VHH (Nanobody) fused to fluorescent protein mCherry.

• Applications: IF, Live Cell Imaging
• Reactivity: mNeonGreen
• Reporter/Conjugate: mCherry
• Vector type: Mammalian expression vector
• Promoter: CMV
• Codon usage: Mammalian
• Selection: Kan/Neo
• Clone#: CTK0234
• Class: Chromobody
• Affinity: 17 kDa
• Molecular Weight: 41.3 kDa
• Type: Chromobody
• Host: Alpaca
• Immunogen: mNeonGreen
• Excitation/Emission Maxima Wavelengths: 587 nm / 610 nm
• Form: Liquid
• Storage buffer: H₂O
• Shipping: ambient temperature
• Storage condition: -20°C
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.