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mNeonGreen Polyclonal antibody

mNeonGreen Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29523-1-AP

Synonyms

blFP-Y3, mNeonGreen

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using mNeonGreen Polyclonal antibody (29523-1-AP)

    WB analysis using 29523-1-AP

    mNeonGreen tagged proteins and Native HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 29523-1-AP (mNeonGreen antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29523-1-AP targets mNeonGreen in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen mNeonGreen fusion protein Ag31617
Full Name mNeonGreen
Calculated Molecular Weight29 kDa
GenBank Accession NumberPF01353
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.

Protocols

Product Specific Protocols
WB protocol for mNeonGreen antibody 29523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity

Authors - Li Wan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Amy (Verified Customer) (03-18-2024)

Not very clean, some non-specific bands, mNG-tagged protein can be observed. HEK293T lysates 25ug.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T