ChromoTek mEos-Trap Magnetic Agarose Kit

The ChromoTek mEos-Trap Magnetic Agarose Kit consists of an anti-mEos Nanobody/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mEos-tagged proteins from cell extracts of various organisms.

Specificity

mEos-Tag (mEos2 and mEos4b)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Cat no : metmak

Synonyms

mEos Nanobody, mEos2, mEos4b

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Validation Data Gallery

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  IP of mEos4b protein from transfected HEK293T cells using mEos-Trap Magnetic Agarose. I: Input, F: Flow-Through, B: Bound.

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Product Information

The ChromoTek mEos-Trap Magnetic Agarose Kit consists of an anti-mEos Nanobody/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mEos-tagged proteins from cell extracts of various organisms.

• Description: The HA-Trap Magnetic Agarose Kit contains mEos-Trap magnetic agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of mEos-tagged proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to members of the mEos-tag family fused to a protein of interest at N-, C- or internal position. To ensure broad coverage of mEos variants, the Nanobody was optimized for binding to both mEos2 and mEos4b, which are representative of the different lineages of the mEos family. There is no cross-reactivity to other fluorescent proteins such as eGFP.
• Binding Capacity: 10 ug of recombinant mEos4b (28 kDa) per 25 uL bead slurry
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 1 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Camelid
• Affinity (KD): 75 nM
• Compatibility with mass spectrometry: The mEos-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3552229
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component	Description
mEos-Trap Magnetic Agarose	20 reactions (500 µl)
Lysis buffer	Optimized for cytoplasmic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.sis
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution