ChromoTek mEos-Trap Agarose

The ChromoTek mEos-Trap Agarose consists of an anti-mEos Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of proteins fused to the mEos-tag from cell extracts of various organisms.

Specificity

mEos-tag (mEos2 and mEos4b)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Cat no : meta

Synonyms

mEos Nanobody, mEos2, mEos4b

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Validation Data Gallery

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  IP of mEos4b protein from transfected HEK29T cells using mEos-Trap Agarose. I: Input, F: Flow-Through, B: Bound.

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Product Information

The ChromoTek mEos-Trap Agarose consists of an anti-mEos Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of proteins fused to the mEos-tag from cell extracts of various organisms.

• Description: Immunoprecipitation of mEos-tagged proteins with anti-mEos Nanobody conjugated to agarose beads.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to members of the mEos-tag family fused to a protein of interest at N-, C- or internal position. To ensure broad coverage of mEos variants, the Nanobody was optimized for binding to both mEos2 and mEos4b, which are representative of the different lineages of the mEos family. There is no cross-reactivity to other fluorescent proteins such as eGFP.
• Binding Capacity: 15 ug of recombinant mEos4b (28 kDa) per 25 ul bead slurry
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 1M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Camelid
• Affinity (KD): 75 nM
• Compatibility with mass spectrometry: The mEos-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3552227
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!