mCherry Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

  Non-transfected and transfected HEK-293 lysates were subjected to SDS PAGE followed by western blot with 68088-1-Ig (mCherry antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (68088-1-Ig, Clone: 1B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

mCherry Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68088-1-PBS

Clone No.1B3C6

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, ELISA

Synonyms

mCherry

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Product Information

68088-1-PBS targets mCherry in WB, IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: mCherry fusion protein Ag25320
• Full Name: mCherry
• Calculated Molecular Weight: 27 kDa
• RRID: AB_2918825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.