m7G Monoclonal antibody

m7G Monoclonal Antibody for IHC, ELISA, Dot Blot

Host / Isotype

Mouse / IgG2b


Human, mouse


IHC, ELISA, Dot Blot





Cat no : 68302-1-Ig


RNA methylation

Tested Applications

Positive IHC detected inmouse testis tissue, human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive ELISA detected incompound
Positive Dot Blot detected inHeLa cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
DOT BLOTDOT BLOT : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68302-1-Ig targets m7G in IHC, ELISA, Dot Blot applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen compound
Full Name m7G
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

7-Methylguanosine (m7G) is a modified purine nucleoside. It is a methylated version of guanosine and when found in human urine, it may be a biomarker of some types of cancer. In the RNAs, 7-methylguanosine have been used to study and examine the reaction evolving methylguanosine. It also plays a role in mRNA as a blocking group at its 5´-end. The m7G modification actively participates in biological and pathological functions by affecting the metabolism of various RNA molecules, including messenger RNA, ribosomal RNA, microRNA, and transfer RNA. Increasing evidence indicates a critical role for m7G in human disease development, especially cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes.


Product Specific Protocols
IHC protocol for m7G antibody 68302-1-IgDownload protocol
Standard Protocols
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