Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 28074-1-AP

Synonyms

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen



Tested Applications

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, mouse spleen tissue
Positive IF-Fro detected inmouse spleen tissue
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:1500-1:6000
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28074-1-AP targets Ki-67 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ki-67 fusion protein Ag27894
Full Name antigen identified by monoclonal antibody Ki 67
Calculated Molecular Weight 351 kDa
GenBank Accession NumberNM_001081117
Gene Symbol ki67
Gene ID (NCBI) 17345
RRIDAB_2918145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Protocols

Product Specific Protocols
IHC protocol for Ki-67 antibody 28074-1-APDownload protocol
IF protocol for Ki-67 antibody 28074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Drug Resist Updat

Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration

Authors - Ning Ji

Cell Mol Immunol

Targeting PHGDH reverses the immunosuppressive phenotype of tumor-associated macrophages through α-ketoglutarate and mTORC1 signaling

Authors - Zhengnan Cai
mouseIF

Biosens Bioelectron

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

Authors - Xiaoyan Lyu
mouseIHC

Cancer Lett

Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma

Authors - Piao Luo
mouseIHC

ACS Appl Mater Interfaces

Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma

Authors - Xueying An
mouseIHC

Phytomedicine

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer

Authors - Meiyang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ruchi (Verified Customer) (06-08-2023)

Stained Ki67 on murine diabetic skin

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse skin
Ki-67 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:28074-1-AP)