Validation Data Gallery
|Positive IHC detected in
|mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|NIH/3T3 cells, mouse spleen tissue, mouse colon tissue
|Positive FC detected in
|IHC : 1:1000-1:4000
|IF : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
28074-1-AP targets ki67 in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ki67 fusion protein Ag27894
|antigen identified by monoclonal antibody Ki 67
|Calculated molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ruchi (Verified Customer) (06-08-2023)
Stained Ki67 on murine diabetic skin