ki67 Polyclonal antibody

ki67 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 28074-1-AP

Synonyms

D630048A14Rik, Ki 67, Ki67, Mki67



Tested Applications

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, mouse spleen tissue, mouse colon tissue
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

28074-1-AP targets ki67 in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ki67 fusion protein Ag27894
Full Name antigen identified by monoclonal antibody Ki 67
Calculated molecular weight 351 kDa
GenBank accession numberNM_001081117
Gene symbol Mki67
Gene ID (NCBI) 17345
RRIDAB_2918145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ki67 antibody 28074-1-APDownload protocol
IF protocol for ki67 antibody 28074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biosens Bioelectron

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

Authors - Xiaoyan Lyu
mouseIHC

Phytomedicine

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer

Authors - Meiyang Chen
mouseIHC

Biochem Pharmacol

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT

Authors - Xing Chang
humanWB,IF

Genes Cells

Local anesthetic lidocaine induces growth suppression of HeLa cells by decreasing and changing the cellular localization of the proliferation marker Ki-67

Authors - Keiko Haraguchi-Suzuki
mouseIHC

J Oncol

EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress

Authors - Wenbing Wu
humanIHC

In Vitro Cell Dev Biol Anim

TRIM47 promotes glioma angiogenesis by suppressing Smad4

Authors - Zifan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruchi (Verified Customer) (06-08-2023)

Stained Ki67 on murine diabetic skin

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse skin
ki67 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:28074-1-AP)