Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment

Conjugate

Unconjugated

Publications

60

Reviews (1)

Cat no : 28074-1-AP

Synonyms

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

---

Validation Data Gallery

• 

  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28074-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28074-1-AP (ki67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using ki67 antibody (28074-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse spleen using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using ki67 antibody (28074-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of NIH/3T3 using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ki67 antibody (28074-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 28074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ki67 antibody (28074-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of NIH/3T3 using 28074-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Mouse ki67 (28074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue, mouse spleen tissue
• Positive IF-Fro detected in: mouse spleen tissue
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:1500-1:6000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 39 publications below
• IF: See 13 publications below

Product Information

28074-1-AP targets Ki-67 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ki-67 fusion protein Ag27894
• Full Name: antigen identified by monoclonal antibody Ki 67
• Calculated Molecular Weight: 351 kDa
• GenBank Accession Number: NM_001081117
• Gene Symbol: ki67
• Gene ID (NCBI): 17345
• RRID: AB_2918145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Publications

Species	Application	Title
mouse	IHC	Drug Resist Updat; Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration; Authors - Ning Ji
		Cell Mol Immunol; Targeting PHGDH reverses the immunosuppressive phenotype of tumor-associated macrophages through α-ketoglutarate and mTORC1 signaling; Authors - Zhengnan Cai
mouse	IF	Biosens Bioelectron; 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing; Authors - Xiaoyan Lyu
mouse	IHC	Cancer Lett; Icaritin with autophagy/mitophagy inhibitors synergistically enhances anticancer efficacy and apoptotic effects through PINK1/Parkin-mediated mitophagy in hepatocellular carcinoma; Authors - Piao Luo
mouse	IHC	ACS Appl Mater Interfaces; Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma; Authors - Xueying An
mouse	IHC	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Ruchi (Verified Customer) (06-08-2023)

Stained Ki67 on murine diabetic skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

Ki-67 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:28074-1-AP)

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011