ChromoTek iST Myc-Trap Kit for AP-MS sample preparation of Myc-fusion proteins

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

Myc-Tag

Applications

IP, MS

Conjugate

Agarose

Type

Nanobody

Cat no : ytak-iST

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

DescriptionThe iST Myc-Trap® Kit enables researchers to process Myc-tagged proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek Myc-Trap for immunoprecipitation/Co-IP of Myc-tagged proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

• Reliable Identification of Myc-tagged proteins & interacting partners by MS. Endogenous c-myc is NOT bound.

• Identification of even low abundant/ low expressed Myc-tagged proteins

• Superior processing of IP samples into clean peptide mixtures

• Intuitive protocol and reproducible results

ApplicationsIP, MS
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding capacity17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe Myc-Trap® is optimized for on-bead digestion.
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store Myc-Trap at 4°C and Digest at -20°C

Kit components

ComponentDescription
Myc-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.