ChromoTek iST Myc-Trap® Kit for AP-MS sample preparation of Myc-fusion proteins

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

Specificity

Myc-Tag

Applications

IP, MS

Conjugate

Agarose

Type

Nanobody

Publications

2

Cat no : ytak-iST

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL

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  Schematic workflow of iST Nano-Trap® Kit

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Product Information

Kit for IP/ Co-IP of Myc-tagged proteins & sample preparation for mass spectrometry (MS)

• Description: The iST Myc-Trap® Kit enables researchers to process Myc-tagged proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek Myc-Trap for immunoprecipitation/Co-IP of Myc-tagged proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

  • Reliable Identification of Myc-tagged proteins & interacting partners by MS. Endogenous c-myc is NOT bound.

  • Identification of even low abundant/ low expressed Myc-tagged proteins

  • Superior processing of IP samples into clean peptide mixtures

  • Intuitive protocol and reproducible results

• Applications: IP, MS
• Specificity/Target: Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
• Binding capacity: 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): 1x Myc-tag: Dissociation constant K_D of 500 nM; 2x Myc-tag: Dissociation constant K_D of 0.5 nM
• Compatibility with mass spectrometry: The Myc-Trap® is optimized for on-bead digestion.
• Storage Buffer: 20% ethanol
• Storage Condition: Kit is shipped at ambient temperature. Upon receipt store Myc-Trap at 4°C and Digest at -20°C

Kit components

Component	Description
Myc-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.

Publications

Application	Title
	bioRxiv; ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer; Authors - Chen Qian
	Nucleic Acids Res; ONECUT2 acts as a lineage plasticity driver in adenocarcinoma as well as neuroendocrine variants of prostate cancer; Authors - Chen Qian