human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10B10

Cat no : 66484-1-PBS

Synonyms

IgM, IGHM, VH, MU, 2D10B10

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Validation Data Gallery

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  WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66484-1-Ig (IGHM antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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Product Information

66484-1-PBS targets human IgM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IgM fusion protein Ag1459
• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).