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Recombinant Human CD38 protein (Myc Tag, His Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0110

Validation Data Gallery

  • Purity of Recombinant Human CD38 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD38 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CD38 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CD38 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD38 protein Val43-Ile300 (Accession# P28907-1) with a Myc tag and a His tag at the C-terminus.
GeneID 952
Accession P28907-1
PredictedSize 34.9 kDa
SDS-PAGE 38-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose. CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis. In addition to the membrane form, CD38 can also exist as a soluble form (sCD38), which is derived from the membrane form by proteolytic cleavage occurring near the cell membrane.

References:

1. Jackson DG. et al. J Immunol. 1990;144(7):2811-2815.
2. Funaro A. et al.1996;8(11):1643-1650.
3. Cho YS. et al. J Biol Chem. 2000;275(3):1685-1690.
4. Lebedev MJ. et al. 2004;30(6):552-556.
5. Schneider M. et al. PLoS One. 2015;10(5):e0126007.