CoraLite®594-conjugated hIST1 Monoclonal antibody

hIST1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E12A7

Cat no : CL594-66989

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-66989

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 hIST1 antibody (CL594-66989, Clone: 1E12A7 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66989 targets hIST1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: hIST1 fusion protein Ag28364
• Full Name: KIAA0174
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000430
• Gene Symbol: hIST1
• Gene ID (NCBI): 9798
• RRID: AB_2920068
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.