Anti-Human gp130/IL6ST Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98121-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98121-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98121-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98121-1-RR against Human gp130/IL6ST were performed. The affinity constant is 115 nM.

gp130/IL6ST {ptg:Brand} Recombinant Antibody for FC

Cat No. 98121-1-RR

Clone No.240992H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL6ST, 240992H1, CD130, Glycoprotein 130, GP130

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98121-1-RR targets gp130/IL6ST in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Calculated Molecular Weight: 104 kDa
• GenBank Accession Number: NM_002184.3
• Gene Symbol: IL6ST
• Gene ID (NCBI): 3572
• RRID: AB_3672268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P40189-1
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3.