Filter: AllWBIHC

WB analysis using 67766-1-Ig (same clone as 67766-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
WB analysis of NCCIT using 67766-1-Ig (same clone as 67766-1-PBS)
NCCIT cells were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

gp130/IL6ST Monoclonal antibody, PBS Only

gp130/IL6ST Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67766-1-PBS

Clone No.1H7C5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67766-1-PBS targets gp130/IL6ST in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	gp130/IL6ST fusion protein Ag28808 Predict reactive species
Full Name	interleukin 6 signal transducer (gp130, oncostatin M receptor)
Calculated Molecular Weight	104 kDa
Observed Molecular Weight	130-140 kDa
GenBank Accession Number	NM_002184
Gene Symbol	IL6ST
Gene ID (NCBI)	3572
RRID	AB_2918533
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P40189
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).