gephyrin Polyclonal antibody, PBS Only

gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12681-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 12681-1-AP (gephyrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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Product Information

12681-1-PBS targets gephyrin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: gephyrin fusion protein Ag3370
• Full Name: gephyrin
• Calculated Molecular Weight: 769 aa, 83 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030016
• Gene Symbol: GPHN
• Gene ID (NCBI): 10243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.