calcyphosine Monoclonal antibody

calcyphosine Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7D1

Cat no : 68225-1-Ig

Synonyms

Calcyphosin, calcyphosine, CAPS, CAPS1

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Validation Data Gallery

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  WB analysis using 68225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit lung using 68225-1-Ig

  rabbit lung tissue were subjected to SDS PAGE followed by western blot with 68225-1-Ig (CAPS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 68225-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using calcyphosine antibody (68225-1-Ig, Clone: 2B7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: SK-BR-3 cells, pig lung tissue, rabbit lung tissue, U-251 cells, PC-3 cells
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68225-1-Ig targets calcyphosine in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: calcyphosine fusion protein Ag10330
• Full Name: calcyphosine
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC080599
• Gene Symbol: calcyphosine
• Gene ID (NCBI): 828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.