CoraLite®594-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, hamster

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

5E10C4

Cat no : CL594-60315

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-60315 (c-SRC antibody) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

• 

  FC experiment of MCF-7 using CL594-60315

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-SRC (CL594-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-60315 targets c-SRC in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, hamster samples.

• Tested Reactivity: human, mouse, hamster
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-SRC fusion protein Ag22306
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• RRID: AB_2883461
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publications

Species	Application	Title
human	IF	Mol Cancer; Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.; Authors - Zhenzhen Pan