Beta Arrestin 1 Monoclonal antibody

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• KD/KO Validated

Beta Arrestin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H8E4

Cat no : 67580-1-Ig

Synonyms

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1

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Validation Data Gallery

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  WB analysis using 67580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67580-1-Ig

  WB result of Beta Arrestin 1 antibody (67580-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Arrestin 1 transfected HepG2 cells.

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  WB analysis of HeLa using 67580-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67580-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 67580-1-Ig

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, NIH/3T3 cells, human peripheral blood platelets, K-562 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67580-1-Ig targets Beta Arrestin 1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beta Arrestin 1 fusion protein Ag7504
• Full Name: arrestin, beta 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC003636
• Gene Symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• RRID: AB_2882791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).