APP Polyclonal antibody

APP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27320-1-AP

Synonyms

amyloid precursor protein, Alzheimer disease amyloid protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42

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Validation Data Gallery

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  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 27320-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HEK-293T cells, U-251 cells, mouse brain tissue, HeLa cells, rat brain tissue, MDA-MB-231 cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

27320-1-AP targets APP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APP fusion protein Ag26299
• Full Name: amyloid beta (A4) precursor protein
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC004369
• Gene Symbol: APP
• Gene ID (NCBI): 351
• RRID: AB_2880842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).

Publications

Species	Application	Title
human	WB	Alzheimers Res Ther; Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.; Authors - Yaqi Wang
mouse	IHC	Brain Res; Investıgatıon of the protectıve effect of long-term exercıse on molecular pathways and behavıours ın scopolamıne ınduced alzheımer's dısease-lıke condıtıon; Authors - Seda Kose