ChromoTek anti-Cystatin-C recombinant VHH, biotinylated

Cyc-b is a recombinant mono-biotinylated anti Cystatin-C Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat no : Cyc-b

Synonyms

Cystatin-C VHH, Cystatin-C single domain antibody (sdAb), CytC, Cyt-C

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Validation Data Gallery

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  BLI analysis of the capture of varying concentrations of HumanKine Cystatin C (HZ-1211) by biotinylated anti-Cystatin C VHH (Cyc-b). Fit indicates fitting of data to a 1:1 binding model (as Cystatin-C is a monomer).

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  ELISA analysis of the capture of varying concentrations of HumanKine Cystatin C (HZ-1211) by biotinylated anti-Cystatin C VHH (Cyc-b). Detection via rabbit polyclonal PTG 12245-1-AP.

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Product Information

Cyc-b is a recombinant mono-biotinylated anti Cystatin-C Nanobody (VHH), suitable for ELISA, SPR, and BLI applications.

• Applications: BLI, SPR, ELISA
• Reactivity: Human
• Conjugate: Biotin
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: 35 nM
• Molecular Weight: 14.7 kDa
• Form: Liquid
• RRID: AB_3665407
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Shipping: ambient
• Background: Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.