androgen receptor Monoclonal antibody, PBS Only

androgen receptor Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7C12

Cat no : 66747-1-PBS

Synonyms

AR, androgen receptor,AR, NR3C4, DHTR, 1F7C12

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Validation Data Gallery

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  WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

  PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66747-1-Ig (same clone as 66747-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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Product Information

66747-1-PBS targets androgen receptor in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: androgen receptor fusion protein Ag17291
• Full Name: androgen receptor
• Calculated Molecular Weight: 914 aa, 99 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC132975
• Gene Symbol: AR
• Gene ID (NCBI): 367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )