Alpha Internexin Polyclonal antibody

Alpha Internexin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23881-1-AP

Synonyms

66 kDa neurofilament protein, Alpha internexin, Alpha Inx, INA, NEF5, Neurofilament 5, NF 66, NF-66, TXBP 1

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Validation Data Gallery

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  WB analysis using 23881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23881-1-AP (alpha-internexin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23881-1-AP (alpha-internexin antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23881-1-AP (alpha-internexin antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 23881-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23881-1-AP targets Alpha Internexin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Internexin fusion protein Ag20844
• Full Name: internexin neuronal intermediate filament protein, alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 66-68 kDa
• GenBank Accession Number: BC006359
• Gene Symbol: INA
• Gene ID (NCBI): 9118
• RRID: AB_2879348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.