Zyxin Monoclonal antibody, PBS Only

Zyxin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D7E7

Cat no : 60254-1-PBS

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2

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Validation Data Gallery

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  WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60254-1-Ig (same clone as 60254-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60254-1-Ig (same clone as 60254-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60254-1-Ig (Zyxin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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Product Information

60254-1-PBS targets Zyxin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Zyxin fusion protein Ag0419
• Full Name: zyxin
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC008743
• Gene Symbol: Zyxin
• Gene ID (NCBI): 7791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.