ZnT3 Monoclonal antibody

ZnT3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9B1

Cat no : 67262-1-Ig

Synonyms

SLC30A3, Zinc transporter 3, ZnT 3, ZNT3

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Validation Data Gallery

• 

  WB analysis of pig brain using 67262-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67262-1-Ig (ZnT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67262-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67262-1-Ig (ZnT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67262-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67262-1-Ig (ZnT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67262-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67262-1-Ig (ZnT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67262-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67262-1-Ig (ZnT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig brain tissue, HEK-293 cells, Jurkat cells, rat brain tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67262-1-Ig targets ZnT3 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ZnT3 fusion protein Ag10735
• Full Name: solute carrier family 30 (zinc transporter), member 3
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC028358
• Gene Symbol: ZnT3
• Gene ID (NCBI): 7781
• RRID: AB_2882533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZnT3, encoded by SLC30A3, is a member of SLC30A family which is zinc transporter sequestrating cytosolic zinc into intracellular compartments or effluxing zinc to the extracellular space. Excessive zinc level can cause neuron apoptosis and lead to cognitive impairments. Znt3 facilitates the transport and storage of zinc in synaptic vesicles. Abnormal ZnT3 expression has been linked to some neurodegenerative disorders.