ZP3 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01801-2

  Cytometric bead array standard curve of MP01801-2, ZP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85084-1-PBS. Detection antibody: 85084-2-PBS. Standard: Ag15791. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85084-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85084-1-RR against Human ZP3 were performed. The affinity constant is below 1 pM.

ZP3 {ptg:Brand} Recombinant Antibody for WB, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 85084-1-PBS

Clone No.242729A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3

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Product Information

85084-1-PBS targets ZP3 as part of a matched antibody pair:

MP01801-2: 85084-1-PBS capture and 85084-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZP3 fusion protein Ag15791
• Full Name: zona pellucida glycoprotein 3 (sperm receptor)
• Calculated Molecular Weight: 373 aa, 41 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC113949
• Gene Symbol: ZP3
• Gene ID (NCBI): 7784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21754
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).