Various lysates were subjected to SDS PAGE followed by western blot with RMX00002 (ZO1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00002 (ZO1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using RMX00002
IP result of anti-ZO1 (IP:RMX00002, 4ug; Detection:RMX00002 1:20000) with HEK-293 cells lysate 1800 ug.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using RMX00002
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using RMX00002
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using RMX00002
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (RMX00002) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (RMX00002) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
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Caco-2 cells, HEK-293 cells, HT-29 cells, HeLa cells
Positive IP detected in
HEK-293 cells
Positive IHC detected in
rat colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00002 targets ZO-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
Various lysates were subjected to SDS PAGE followed by western blot with RMX00002 (ZO1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse colon using RMX00002
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using RMX00002
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using RMX00002
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using RMX00002 (ZO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using RMX00002
IP result of anti-ZO1 (IP:RMX00002, 4ug; Detection:RMX00002 1:20000) with HEK-293 cells lysate 1800 ug.
IF/ICC Figures
IF Staining of MCF-7 using RMX00002
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (RMX00002) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ZO-1 Multi-Recombinant antibody
Catalog Number
RMX00002
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:500-1:2000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
