ZO-1 Polyclonal antibody

ZO-1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 30487-1-AP

Synonyms

DKFZp686M05161, Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, Zona occludens protein 1, Zonula occludens protein 1

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Validation Data Gallery

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  WB analysis using 30487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30487-1-AP (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 30487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-1 antibody (30487-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HT-29 cells, HUVEC cells, HepG2 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

30487-1-AP targets ZO-1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZO-1 fusion protein Ag33182
• Full Name: tight junction protein 1 (zona occludens 1)
• Calculated Molecular Weight: 1748 aa, 195 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC111712
• Gene Symbol: ZO-1
• Gene ID (NCBI): 7082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Mol Med; 5α-Hydroxycostic acid inhibits choroidal neovascularization in rats through a dual signalling pathway mediated by VEGF and angiopoietin 2; Authors - Wulong Lei
human	WB	Cells; The Natural Product Secoemestrin C Inhibits Colorectal Cancer Stem Cells via p38-S100A8 Feed-Forward Regulatory Loop; Authors - Huimin Zhou