ZO-2 Recombinant antibody, PBS Only

ZO-2 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230474D1

Cat no : 83111-1-PBS

Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, Zona occludens protein 2, Zonula occludens protein 2

---

Validation Data Gallery

• 

  WB analysis using 83111-1-RR (same clone as 83111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-1-RR (ZO-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83111-1-RR (same clone as 83111-1-PBS)

  WB result of ZO-2 antibody (83111-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83111-1-PBS targets ZO-2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZO-2 fusion protein Ag13534
• Full Name: tight junction protein 2 (zona occludens 2)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: ~150 kDa
• GenBank Accession Number: BC027592
• Gene Symbol: TJP2
• Gene ID (NCBI): 9414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.