ZNF828 Polyclonal antibody

ZNF828 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26315-1-AP

Synonyms

Chromosome alignment-maintaining phosphoprotein 1, CHAMP1, CHAMP, CAMP, C13orf8

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Validation Data Gallery

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  WB analysis of Jurkat using 26315-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26315-1-AP (ZNF828 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 26315-1-AP

  IP result of anti-ZNF828 (IP:26315-1-AP, 4ug; Detection:26315-1-AP 1:1000) with Jurkat cells lysate 4000ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26315-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26315-1-AP (ZNF828 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26315-1-AP (ZNF828 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF828 antibody (26315-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26315-1-AP targets ZNF828 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF828 fusion protein Ag23698
• Full Name: zinc finger protein 828
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC065237
• Gene Symbol: ZNF828
• Gene ID (NCBI): 283489
• RRID: AB_2880476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.