ZNF8 Polyclonal antibody

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17448-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 17448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 17448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 17448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 17448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17448-1-AP targets ZNF8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF8 fusion protein Ag11571
• Full Name: zinc finger protein 8
• Calculated Molecular Weight: 575 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC039323
• Gene Symbol: ZNF8
• Gene ID (NCBI): 7554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.