ZNF750 Polyclonal antibody

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  IP experiment of SiHa using 21752-1-AP

  IP result of anti-ZNF750 (IP:21752-1-AP, 4ug; Detection:21752-1-AP 1:500) with SiHa cells lysate 1520 ug.

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  IHC staining of human oesophagus cancer using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21752-1-AP (ZNF750 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21752-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21752-1-AP (ZNF750 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZNF750 Polyclonal Antibody for IHC, IP, ELISA

Cat No. 21752-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Protein ZNF750, Zfp750, Zinc finger protein 750

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Tested Applications

• Positive IP detected in: SiHa cells
• Positive IHC detected in: human oesophagus cancer tissue, human skin tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

21752-1-AP targets ZNF750 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF750 fusion protein Ag16116
• Full Name: zinc finger protein 750
• Calculated Molecular Weight: 723 aa, 77 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC109037
• Gene Symbol: ZNF750
• Gene ID (NCBI): 79755
• RRID: AB_10863125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q32MQ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF750 is a novel nuclear protein highly expressed in differentiated skin keratinocytes. Mutation of ZNF750 is linked with familial psoriasis. Recently ZNF was found to be significantly mutated in esophageal squamous cell carcinoma (ESCC). Expression of ZNF750 is decreased in ESCC compared to the normal tissue.

Publications

Species	Application	Title
human	IHC	Int J Cancer; Identification of calmodulin-like protein 5 as tumor-suppressor gene silenced during early stage of carcinogenesis in squamous cell carcinoma of uterine cervix.; Authors - Sohei Kitazawa
human	WB	Oncol Lett; Differentiation-related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma.; Authors - Sheyne Sta Ana Choi
human	WB	Cell Death Discov; TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750; Authors - Qiyi Yi
human,mouse	WB	Cell Death Dis; GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma; Authors - Jiaru Hu