ZNF658 Polyclonal antibody

ZNF658 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24823-1-AP

Synonyms

zinc finger protein 658, ZNF658

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Validation Data Gallery

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  WB analysis of fetal human brain using 24823-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24823-1-AP (ZNF658 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24823-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24823-1-AP (ZNF658 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24823-1-AP (ZNF658 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 24823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24823-1-AP (ZNF658 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24823-1-AP is ZNF658 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF658 fusion protein Ag20508
• Full Name: zinc finger protein 658
• Calculated Molecular Weight: 1059 aa, 122 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC031626
• Gene Symbol: ZNF658
• Gene ID (NCBI): 26149
• RRID: AB_2879744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.