ZNF649 Recombinant antibody

ZNF649 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242036C7

Cat no : 84624-4-RR

Synonyms

Zinc finger protein 649, 242036C7

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Validation Data Gallery

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  WB analysis using 84624-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84624-4-RR (ZNF649 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84624-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84624-4-RR against Human ZNF649 were performed. The affinity constant is 4.71 nM.

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, HCT116 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84624-4-RR targets ZNF649 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZNF649 fusion protein Ag22700
• Full Name: zinc finger protein 649
• Calculated Molecular Weight: 505 aa, 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC005368
• Gene Symbol: ZNF649
• Gene ID (NCBI): 65251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF649, also named as Zinc finger protein 649, is a 505 amino acid protein, which contains The one KRAB domain and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF649 is highly expressed in heart, skeletal muscle, and brain and lower expression in liver, lung, kidney, pancreas and placenta. ZNF649 as a regulator of transcriptional factor complexes may suppress SRE and AP-1 transcription activities by growth factor signaling pathways.